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Laboratory Forage Analysis
by Dr. Don Ball

As discussed in an article in Cooperative Farming News last month, sensory evaluation of hay (via sight, touch, smell, etc.) can be useful, but the fact is that accurate laboratory testing of feed and forage is required to provide the information needed to formulate animal rations. Furthermore, laboratory testing to assess quality also provides a highly reliable basis for commercial hay sales.

A typical laboratory forage analysis includes measurements of dry matter, crude protein, and fiber (acid detergent fiber and neutral detergent fiber). Sometimes ash is measured, and when heat-damaged protein is suspected it may be desirable to measure acid detergent insoluble crude protein. Some additional results that may be provided on laboratory reports are also measured, while others such as digestible energy or protein, net energy, total digestible nutrients, potential intake, etc., are calculated or estimated from measured analyses.

Dry matter -  Dry matter (DM) is the portion (weight) of forage other than water. Nutrients are typically reported on a DM basis to eliminate the dilution effect of moisture and to allow more direct comparison of feeds and easier formulation of diets. To compare prices and nutritive value between or among lots of forage, they should be adjusted to a DM basis. Sometimes hay is compared or sold on a 90% DM basis, which closely resembles the average DM of air dried feeds.

For hay, excessively low moisture (less than 10%) could indicate brittleness (and thus low palatability) or excessive leaf loss (which is linked with lowered forage quality). High moisture (greater than 14 to 18%) indicates a risk of mold. For silage, excessively low moisture (below 45%) can indicate heat damage, while high moisture (above 70%) is often associated with poor fermentation and potential intake problems.

Detergent fiber analysis - Acid detergent fiber (ADF) and neutral detergent fiber (NDF) are commonly used as standard forage testing techniques for fiber analysis. NDF approximates the total cell wall constituents including hemi-cellulose, whereas ADF primarily represents cellulose and lignin. ADF is often used to calculate digestibility, and NDF is used to predict intake potential. As fiber increases, forage quality declines.

Protein - Protein is a key nutrient that must be considered both in amount and type for various animal diets. It is commonly measured as crude protein (CP), which is 6.25 times the nitrogen content of forage. Crude protein is used rather than just "protein" because rumen microbes can convert non-protein nitrogen in microbial protein, which can then be used by the animal. However, this value should be used with some care, as it is not applicable to non-ruminants or when high levels of nitrate are present in the forage.

High-performing animals, especially milking dairy cows, need larger amounts of protein to be absorbed from the intestines than rumen microbes produce. Therefore, they need a certain amount of "rumen bypass protein" (or RUP) in the ration. Recently, calibrations have been developed that allow RUP in forages to be estimated using near infrared reflectance spectroscopy, which is a quick and easy measurement technique.

Digestible energy estimates -  Energy is typically the most limiting nutritional factor on livestock farms and thus is usually of particular interest and importance. However, energy values can be determined directly only by feeding trials. Therefore, laboratory reports provide calculated values of energy. The most common has been to measure a single fiber fraction (usually ADF) and use it to calculate an estimated digestibility, total digestible nutrients (TDN) or net energy for lactation (NE1).

Conclusion

The ultimate measure of forage quality is animal performance. In fact, perhaps the best succinct definition of forage quality is: the extent to which a forage results in desired animal performance (usually some combination of daily gain, reproduction, milk production, or fiber production). It is not necessary for a livestock producer to understand how forage quality is measured in a laboratory, but some understanding of how forage quality affects animals is a basic requirement for obtaining good animal performance.

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Date Last Updated January, 2006